■ 基本信息
別名: | pET35b |
啟動(dòng)子: | T7 |
復(fù)制子: | pBR322 |
終止子: | T7 terminator |
質(zhì)粒分類: | 大腸桿菌載體；PET系列表達(dá)質(zhì)粒 |
質(zhì)粒大小: | 5927bp |
質(zhì)粒標(biāo)簽: | N-CBDclos,C-6×His |
原核抗性: | Kan |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，有氧，LB |
表達(dá)宿主: | BL21(DE3) |
誘導(dǎo)方式: | IPTG或乳糖及其類似物 |
5'測(cè)序引物: | T7:TAATACGACTCACTATAGGG |
3'測(cè)序引物: | T7-ter:TGCTAGTTATTGCTCAGCGG |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 大腸桿菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Kan |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
The pET-35b(+) vector is designed for expression of CBDclos fusion proteins.A variety of cloning sites and strategies are available. Rapid directional cloning of PCR-ampli?ed DNA is available with the pET-35 Xa/LIC Vector Kit, which contains linearized pET-35b(+) ready for annealing with appropriately prepared inserts. When cloned with the Xa/LIC method, resulting CBDclos?Tag? fusion proteins can be cleaved precisely at the vector-encoded junction using Factor Xa. Unique sites are shown on the circle map. Note that the sequence is num-bered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK173pET-35b大腸表達(dá)質(zhì)粒.txt
