■ 基本信息
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
終止子: | BGH poly（A）signal |
質(zhì)粒分類: | 哺乳系列質(zhì)粒；哺乳表達(dá)質(zhì)粒；pCDNA系列質(zhì)粒 |
質(zhì)粒大小: | 5541bp |
質(zhì)粒標(biāo)簽: | N-3×HA |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，5%CO2 |
表達(dá)宿主: | 293T等哺乳細(xì)胞 |
誘導(dǎo)方式: | 無(wú)需誘導(dǎo) |
5'測(cè)序引物: | pCDNA3.1-F（CTAGAGAACCCACTGCTTAC） |
3'測(cè)序引物: | pCDNA3.1-R（TAGAAGGCACAGTCGAGG） |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | G418
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡(jiǎn)介
pCDNA3.1-3×HA-N質(zhì)粒是一種高效表達(dá)的哺乳質(zhì)粒。該質(zhì)粒含有Amp抗性，可轉(zhuǎn)至DH5α中轉(zhuǎn)化。培養(yǎng)條件為L(zhǎng)B培養(yǎng)基，37℃。
pCDNA3.1-3×HA-N vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin selectable marker and a forward-orientation multiple cloning site.High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells Neomycin resistance gene for selection of stable cell lines. There are three HA tags on the N side.Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen.
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK354pCDNA3.1-3×HA-N哺乳表達(dá)質(zhì)粒.txt
