■ 基本信息
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 哺乳細(xì)胞，熒光蛋白報(bào)告載體 |
質(zhì)粒大小: | 4715bp |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG
|
3'測(cè)序引物: | Sv40-polyA-R：GAAATTTGTGATGCTATTGC |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: |
|
原核抗性: | Kan |
真核抗性: | G418
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡(jiǎn)介
pTagBFP-N is a mammalian expression vector encoding blue fluorescent protein TagBFP. The vector allows generation of fusions to the TagBFP N-terminus and expression of TagBFP fusions or TagBFP alone in eukaryotic (mammalian) cells.
TagBFP codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996]. To increase mRNA translation efficiency, Kozak consensus translation initiation site is generated upstream of the TagBFP coding sequence [Kozak, 1987]. Multiple cloning site (MCS) is located between PCMV IE and TagBFP coding sequence。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK456pTagBFP-N哺乳熒光質(zhì)粒.txt
