■ 基本信息
復制子: | pUC |
原核抗性: | Amp |
真核抗性: | Hyg |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
■ 質粒屬性
質粒宿主: | 哺乳細胞 |
質粒用途: | 信號報告 |
片段類型: | Promoter
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | Hyg
|
熒光標記: | Fluc
|
■ 質粒簡介
The pGL4.30[luc2P/NFAT-RE/Hygro] Vector(a–f) contains an NFAT response element (NFAT-RE) that drives the transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than the protein encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK480pGL4.30[luc2P-NFAT-RE-Hygro] 哺乳報告質粒.txt
