■ 基本信息
啟動子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質粒分類: | 哺乳細胞，信號通路報告載體 |
質粒大小: | 4281bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物: | RVP3:CTAGCAAAATAGGCTGTCCC |
3'測序引物: | 根據(jù)序列設計引物 |
■ 質粒屬性
質粒宿主: | 哺乳細胞 |
質粒用途: | 信號報告 |
片段類型: | Promoter |
片段物種: |
|
原核抗性: | Amp |
真核抗性: |
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熒光標記: | Rluc
|
■ 質粒簡介
The pGL4.75[hRluc/CMV] Vector(a-c) encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.75[hRluc/CMV] Vector contains the hRluc reporter gene and a CMV immediate-early enhancer/promoter and can be used as an expression control or a co-reporter vector.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK485pGL4.75[hRluc-CMV]哺乳報告質粒.txt
