■ 基本信息
啟動子: | CMV |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 哺乳細胞，熒光蛋白報告載體 |
質(zhì)粒大小: | 4918bp |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導(dǎo)方式: | 無須誘導(dǎo)，瞬時表達 |
5'測序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG
|
3'測序引物: | 根據(jù)序列設(shè)計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細胞 |
質(zhì)粒用途: | 定位 |
片段類型: |
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片段物種: |
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原核抗性: | Kan |
真核抗性: | G418
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熒光標(biāo)記: |
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■ 質(zhì)粒簡介
pEYFP-Golgi encodes a fusion protein consisting of enhanced yellow fluorescent protein (EYFP) and a sequence encoding the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase (GT). This region of human beta 1,4-GT contains the membrane-anchoring signal peptide that targets the fusion protein to the trans-medial region of the Golgi apparatus. The EYFP gene contains the four amino acid substitutions previously published as GFP-10C. The fluorescence excitation maximum of EYFP is 513 nm; the emission spectrum has a peak at 527 nm (in the yellow-green region). When excited at 514 nm, the Em of EYFP is 84,500 cm-1M-1 and the fluorescence quantum yield is 0.61, resulting in a bright fluorescent signal. The fluorescence observed is roughly equivalent to that of EGFP.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK533pEYFP-Golgi高爾基體定位質(zhì)粒.txt
