■ 基本信息
別名: | Plasmid #63592 |
啟動(dòng)子: | EF-1α core promoter |
復(fù)制子: | Ori，F(xiàn)1 ori，SV40 ori |
終止子: | WPRE |
質(zhì)粒分類: | 哺乳細(xì)胞質(zhì)粒；哺乳編輯質(zhì)粒；哺乳Cas9質(zhì)粒 |
質(zhì)粒大小: | 13177bp |
質(zhì)粒標(biāo)簽: | C-nucleoplasmin NLS，C-FLAG，C-EGFP |
原核抗性: | Amp |
真核抗性: | Zeo |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 293T等哺乳細(xì)胞 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 基因編輯 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | Zeo
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.
LentiCas9-EGFP質(zhì)粒的參考文獻(xiàn)：Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK681LentiCas9-EGFP哺乳編輯質(zhì)粒.txt
