■ 基本信息
復(fù)制子: | pUC |
原核抗性: | Amp |
| 克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞,逆病毒 |
質(zhì)粒用途: | 輔助包裝 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
The pCL-Ampho packaging vector is a part of the RetroMax expression system (Cat#10040K) and has been designed to maximize recombinant–retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of 1 infectious particle/cell/day. Retrovirus tropism is determined at three levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. The plasmid DNA can be amplified by transforming bacteria and growing them on LB/ampicillin (100 mg/ml) media.
