■ 基本信息
啟動子: | CMV |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 病毒系列，慢病毒克隆質(zhì)粒 |
質(zhì)粒大小: | 8102bp |
原核抗性: | Amp |
篩選標(biāo)記: | Puro |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37℃ |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無須誘導(dǎo)，瞬時表達(dá) |
5'測序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG
|
3'測序引物: | 根據(jù)序列設(shè)計引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞,慢病毒 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: | 嘌呤霉素
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熒光標(biāo)記: |
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■ 質(zhì)粒簡介
pLVX-Puro is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to express your gene of interest in virtually any cell type, even primary cells. Expression of your gene is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE), located just upstream of the multiple cloning site (MCS), allowing constitutive, high level expression of your protein of interest.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK756pLVX-Puro慢病毒表達(dá)質(zhì)粒.txt
