■ 基本信息
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
質(zhì)粒大小: | 7567bp |
原核抗性: | Amp |
真核抗性: | EGFP |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞,慢病毒 |
質(zhì)粒用途: | 基因沉默 |
片段類型: | shRNA
|
片段物種: |
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: | 綠色
|
■ 質(zhì)粒簡(jiǎn)介
EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
（pSico shRNA cloning protocol點(diǎn)擊下載）
pSicoshRNAcloningprotocol.pdf
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK826pSicoR慢病毒干擾質(zhì)粒.txt
