■ 基本信息
啟動(dòng)子: | 35s |
復(fù)制子: | pUC |
終止子: | NOS |
質(zhì)粒分類(lèi): | 植物系列，RNAi載體 |
質(zhì)粒大小: | 12880bp |
原核抗性: | Kan |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 植物細(xì)胞 |
5'測(cè)序引物: | 35S:GACGCACAATCCCACTATCC |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 植物 |
質(zhì)粒用途: | 基因沉默 |
片段類(lèi)型: | shRNA |
片段物種: |
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原核抗性: | Kan |
真核抗性: | G418
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
siRNA) caused by RNA (interference RNA short)Interference (RNAi) is a very effective test method. It is a creature The body of a molecule by double stranded RNA (dsRNA) molecules to make specific Degradation of the sequence of mRNA resulted in the silencing of gene expression. This phenomenon occurs at the post transcriptional level, that is, sequence specific Post transcriptional gene silencing.
