■ 基本信息
啟動(dòng)子: | PH |
復(fù)制子: | pUC |
終止子: | polyhedrin 3 |
質(zhì)粒分類: | 昆蟲細(xì)胞載體；桿狀病毒組成型分泌表達(dá)質(zhì)粒 |
質(zhì)粒大小: | 9761bp |
質(zhì)粒標(biāo)簽: | N-GP67 signal |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 昆蟲細(xì)胞 |
誘導(dǎo)方式: | 組成性表達(dá)，無需誘導(dǎo) |
5'測(cè)序引物: | Polyhedrin-F：AAATGATAACCATCTCGC |
3'測(cè)序引物: | Polyhedrin-R：GTCCAAGTTTCCCTG |
備注: | 含有GP67分泌信號(hào)肽 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 昆蟲細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
The acidic glycoprotein gp67 (syn.: gp64) is the most abundant envelope surface glycoprotein of the Autographa californica nuclear polyhedrosis virus (AcNPV baculovirus), and is essential for the entry of baculovirus particles into susceptible insect cells. Since large amounts of this protein are secreted and anchored to the virus peplomer, its gene contains one of the most effective baculovirus-encoded signal sequences for protein secretion. Therefore, we have constructed baculovirus transfer vectors that contain the gp67 signal sequence in front of a multiple cloning site. A gene of choice can be inserted in one of these cloning sites and the protein of interest will be expressed as a gp67 signal peptide fusion protein under the control of the strong baculovirus polyhedrin promoter. This strategy allows the forced secretion of otherwise non-secreted recombinant proteins which may be easily purified when serum-free insect culture medium, BD BaculoGold Max-XP Insect Cell Medium is used. The transfer vector(s) should be preferentially used in conjunction with BD BaculoGold DNA .
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK1044pAcGP67A昆蟲分泌質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
