■ 基本信息
啟動(dòng)子: | PnisA |
復(fù)制子: | repA，repC |
終止子: | T |
質(zhì)粒分類: | 乳酸乳球菌表達(dá)載體 |
質(zhì)粒大小: | 3167bp |
原核抗性: | Chl |
真核抗性: | Chl |
克隆菌株: | MC1061 |
培養(yǎng)條件: | 30度 |
表達(dá)宿主: | NZ9000乳酸乳球菌 |
培養(yǎng)條件: | M17+0.5%葡萄糖/30度密封靜置液體培養(yǎng) |
5'測(cè)序引物: | 根據(jù)序列設(shè)計(jì) |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 乳酸菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: |
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片段物種: |
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原核抗性: | Chl
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真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
The pNZ8148 vector contains an origin of replication (ORI), the gene for the resistance to chloramphenicol, two genes for the replication proteins repA and repC, the nisin-inducible promoter (P nisA), and the transcription terminator (T). The gene, tagged with a Strep-tag II (STREP) followed by a stop codon (*), can be inserted between the necessary NcoI site and another endonuclease site from the multicloning site (MCS) such as PstI, SphI, KpnI, SpeI, XbaI, SacI and HindIII. The replicons of the vectors pNZ8008, pNZ8148, pNZ8149 and pNZ8150 are identical and came originally from the Lactococcus lactis plasmid pSH71. However, this replicon has a broad host range. Plasmids with this replicon can replicate in many Gram-positive bacteria, such as Lactobacillus plantarum and Streptococcus thermophilus. pNZ8148 – In this vector the nisA promoter is followed by an NcoI site for translational fusions at the ATG. It contains a terminator after the MCS. Sequence adaptation for cloning in NcoI can result in a change in the second amino acid of a protein (Mierau and Kleerebezem, 2005).
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK1060pNZ8148乳酸菌分泌質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
