■ 基本信息
啟動子: | CYC1 |
復制子: | pBS |
原核抗性: | Kan |
真核抗性: | LEU2 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
■ 質粒屬性
質粒宿主: | 酵母菌 |
質粒用途: | 雜交 |
片段類型: |
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片段物種: |
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原核抗性: | Kan |
真核抗性: | LEU2
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熒光標記: |
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■ 質粒簡介
pBT3-N是一個膜蛋白酵母雙雜交質粒。
■ 質粒圖譜
■ 質粒序列
質粒部分測序序列：
1 tagtacgacg atcttcttag gggcagacat tagaatggta tatccttgaa atatatatat
61 atatattgct gaaatgtaaa aggtaagaaa agttagaaag taagacgatt gctaaccacc
121 tattggaaaa aacaataggt ccttaaataa tattgtcaac ttcaagtatt gtgatgcaag
181 catttagtca tgaacgcttc tctattctat atgaaaagcc ggttccggcg ctctcacctt
241 tcctttttct cccaattttt cagttgaaaa aggtatatgc gtcaggcgac ctctgaaatt
301 aacaaaaaat ttccagtcat cgaatttgat tctgtgcgat agcgcccctg tgtgttctcg
361 ttatgttgag gaaaaaaata atggttgcta agagattcga actcttgcat cttacgatac
421 ctgagtattc ccacagttaa ctgcggtcaa gatatttctt gaatcaggcg ccttagaccg
481 ctcggccaaa caaccaatta cttgttgaga aatagagtat aattatccta taaatataac
541 gtttttgaac acacatgaac aaggaagtac aggacaattg attttgaaga gaatgtggat
601 tttgatgtaa ttgttgggat tccattttta ataaggcaat aatattaggt atgtagatat
661 actagaagtt ctcctcgacc ggtcgatatg cggtgtgaaa taccgcacag atgcgtaagg
721 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
781 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
841 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
901 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
961 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
1021 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
1081 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaggt gtagcggtca
1141 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
1201 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
1261 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
1321 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
1381 cgaattgggt accggccgca aattaaagcc ttcgagcgtc ccaaaacctt ctcaagcaag
1441 gttttcagta taatgttaca tgcgtacacg cgtctgtaca gaaaaaaaag aaaaatttga
1501 aatataaata acgttcttaa tactaacata actataaaaa aataaatagg gacctagacc
1561 ttcaggttgt ctaactcctt ccttttcggt tagagcggag ggtggggggg aggggcgtgt
1621 atgtaagcgt gacataacta attacatgac ctattaagat ctgacgtcag cgctccgcgg
1681 ttagctactt accatggggg ccaaagcggc cttaattaaa ggcctggccg taatggccct
1741 gcaggaattc aaaatcaagc ttatcgaacc ggcc
