■ 基本信息
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal,hGH poly(A) signal |
質(zhì)粒分類: | 哺乳系列質(zhì)粒；哺乳表達(dá)質(zhì)粒；pCMV系列質(zhì)粒 |
質(zhì)粒大小: | 6299bp |
質(zhì)粒標(biāo)簽: | N-3×FLAG |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 293T等哺乳細(xì)胞 |
培養(yǎng)條件: | 37℃，5%CO2 |
誘導(dǎo)方式: | 無須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測序引物:
| CMV-F（CGCAAATGGGCGGTAGGCGTG） |
3'測序引物: | hGH-PA-R（CCAGCTTGGTTCCCAATAGA） |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | G418
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡介
p3×FLAG-CMV-10表達(dá)載體是用于在哺乳動(dòng)物細(xì)胞中建立N-3×FLAG融合蛋白的瞬時(shí)或穩(wěn)定細(xì)胞內(nèi)的pCMV5表達(dá)載體的升級(jí)版。該載體在多克隆區(qū)域上游編碼三個(gè)相鄰的FLAG表位（Asp-Tyr-Lys-Xaa-Xaa-Asp）。這導(dǎo)致使用FLAG抗體的檢測靈敏度增加。第三個(gè)FLAG表位包括腸激酶識(shí)別序列，允許從純化的融合蛋白切割3×FLAG肽。
The p3×FLAG-CMV-10 Expression Vector is a 6.3 kb derivative of pCMV5 used to establish transient or stable intracellular expression of N-terminal 3XFLAG fusion proteins in mammalian cells. The vector encodes three adjacent FLAG? epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein. Efficiency of replication is optimal when using an SV40 T antigen expressing host.
p3×FLAG-CMV-10 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigenexpressing
host, such as COS cells.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK1697 p3×Flag-CMV-10哺乳表達(dá)質(zhì)粒.txt
